期刊
JOURNAL OF DENTAL RESEARCH
卷 93, 期 2, 页码 183-188出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/0022034513513026
关键词
stem cells; periodontitis; injection; inflammation; immune; rats
资金
- National Basic Research Program of China [2010CB944801]
- National Natural Science Foundation of China [81271101, 81070843]
- Program for New Century Excellent Talents in University [NCET-12-0611]
- Beijing Municipal Science and Technology Commission [Z1211000 05212004]
- High-level Talents of Beijing Health System [2013-3-035]
In this study, we investigated the possibility of using local administration of allogeneic bone marrow mesenchymal stem cells (BMMSCs) to induce tissue regeneration in periodontal defects in a rat model of periodontitis. BMMSCs isolated from rats were mixed with 0.9% NaCl solution and injected into periodontal defects. Control groups were 0.9% NaCl solution or left untreated. The clinical assessments, x-rays, and histological examinations were used to evaluate the effect. At 12 wks post-transplantation, quantitative analysis revealed average probing bone loss values of 1.2 +/- 0.19, 1.6 +/- 0.2, and 1.7 +/- 0.14; the bone regeneration rate was 53%, 45%, and 44% in the BMMSC+NaCl group, NaCl group, and untreated group, respectively. The clinical assessments, x-rays, and histological examinations revealed significant periodontal tissue regeneration in the BMMSC injection group, compared with the control groups. The ELISA results showed that TNF, IFN, and IL1 were 2,674.88 +/- 102.77 pg/mL vs. 3,422.1 +/- 51.98 pg/mL, 609.85 +/- 25.5 pg/mL vs. 803.79 +/- 33.85 pg/mL, and 1,038.46 +/- 76.29 pg/mL vs. 1,175.26 +/- 105.55 pg/mL in the BMMSC+NaCl group and NaCl group, respectively, indicating that BMMSC injection inhibited the inflammatory factors TNF, IFN, and IL1. Our results indicate that local administration of BMMSCs can repair defects due to periodontitis, exerting anti-inflammatory and immunomodulatory functions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据