期刊
JOURNAL OF DENTAL RESEARCH
卷 91, 期 1, 页码 84-89出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/0022034511423399
关键词
amelogenin; small exons; evolutionary origin; PCR; enamel; tetrapods
资金
- Centre National de la Recherche Scientifique
- Universite Pierre et Marie Curie [UMR7138]
Amelogenin gene organization varies from 6 exons (1,2,3,5,6,7) in amphibians and sauropsids to 10 in rodents. The additional exons are exons 4, 8, 9, and 4b, the latter being as yet unidentified in AMELX transcripts. To learn more about the evolutionary origin of these exons, we used an in silico approach to find them in 39 tetrapod genomes. AMEL organization with 6 exons was the ancestral condition. Exon 4 was created in an ancestral therian (marsupials + placentals), then exon 9 in an ancestral placental, and finally exons 4b and 8 in rodents, after divergence of the squirrel lineage. These exons were either inactivated in some lineages or remained functional: Exon 4 is functional from artiodactyls onward; exon 9 is known, to date, only in rodents, but could be coding in various mammals; and exon 4b was probably coding in some rodents. We performed PCR of cDNA isolated from mouse and human tooth buds to identify the presence of these transcripts. A sequence analogous to exon 4b, and to exon 9, could not be amplified from the respective tooth cDNA, indicating that even though sequences similar to these exons are present, they are not transcribed in these species.
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