期刊
JOURNAL OF DENTAL RESEARCH
卷 89, 期 12, 页码 1439-1443出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/0022034510378684
关键词
Periostin; periodontal ligament; transforming growth factor beta 1; focal adhesion kinase; matricellular proteins
资金
- Canadian Foundation [18742]
- Canadian Institutes of Health Research IMHA [IMH-94010]
Recently identified as a key component of the murine periodontal ligament (PDL), periostin has been implicated in the regulation of collagen fibril-logenesis and fibroblast differentiation. We investigated whether periostin protein is expressed in the human PDL in situ and the mechanisms regulating periostin expression in PDL fibroblasts in vitro. With immunohistochemistry, periostin protein was identified in the PDL, with expression lower in teeth with reduced occlusal loading. In vitro application of uniaxial cyclic strain to PDL fibroblasts elevated periostin mRNA levels, depending on the age of the patient. Treatment with transforming growth factor-beta1 (TGF-beta 1) also significantly increased periostin mRNA levels, an effect attenuated by focal adhesion kinase (FAK) inhibition. FAK-null fibroblasts contained no detectable periostin mRNA, even after stimulation with cyclic strain. In conclusion, periostin protein is strongly expressed in the human PDL. In vitro, periostin mRNA levels are modulated by cyclic strain as well as TGF-beta 1 via FAK-dependent pathways.
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