期刊
JOURNAL OF DENTAL RESEARCH
卷 88, 期 4, 页码 339-344出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/0022034509334070
关键词
dental pulp cells; tumor necrosis factor-alpha; dentin sialophosphoprotein; matrix metalloproteinase-1; p38 MAPK
资金
- CAPES Foundation Fellowship [0668/07-9]
- [NIH-R01-13725]
Dental pulp cells can differentiate toward an odontoblastic phenotype to produce reparative dentin beneath caries lesions. However, the mechanisms involved in pulp cell differentiation under pro-inflammatory stimuli have not been well-explored. Thus, we hypothesized that the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) could be a mediator involved in dental pulp cell differentiation toward an odontoblastic phenotype. We observed that TNF-alpha-challenged pulp cells exhibited increased mineralization and early and increased expression of dentin phosphoprotein (DPP), dentin sialoprotein (DSP), dentin matrix protein-1, and osteocalcin during a phase of reduced matrix metalloproteinase (MMP) expression. We investigated whether these events were related and found that p38, a mitogen-activated protein kinase, differentially regulated MMP-1 and DSP/DPP expression and mediated mineralization upon TNF-alpha treatment. These findings indicate that TNF-alpha stimulates differentiation of dental pulp cells toward an odontoblastic phenotype via p38, while negatively regulating MMP-1 expression.
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