期刊
JOURNAL OF DAIRY SCIENCE
卷 101, 期 10, 页码 8767-8777出版社
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2018-14655
关键词
lateral flow immunoassay; competitive and sandwich model; aflatoxin M-1; Escherichia coli; O157:H7
资金
- National Natural Science Foundation of China [31772066]
- Jiangxi Special Fund for Agro-Scientific Research in the Collaborative Innovation (China) [JXXTCX201703-1]
- earmarked fund for Jiangxi Agriculture Research System (China) [JXARS-03]
Pathogens, mycotoxins, or antibiotics may exist in a food sample. Micro- and macromolecular substances must be detected quickly. A rapid and convenient lateral flow immunoassay (LFI) integrated with competitive and sandwich models was developed to detect micro- and macromolecular substances. In this study, aflatoxin M-1 (AFM(1)) and Escherichia coil O157:H7 were selected as the micro- and macromolecular substances, respectively. Two test lines in the LFI test strip were evaluated to detect AFM1 and E. coil O157:H7 by competitive and sandwich models. Results showed that the limits of detection for detecting AFM1 and E. coli O157:H7 were 50 pg.mL(-1) and 1.58 x 10(4) cfu.mL(-1), respectively. The whole assay time was 30 min. The recoveries of gold nanoparticle-LFI ranged from 78.0 to 111.6% with coefficients of variation in the range of 3.9 to 8.5% for the detection of AFM(1). For the detection of E. coli O157:H7, the range of recoveries was from 70.1 to 89.6% with coefficients of variation ranging from 4.9 to 13.0%. This study not only tested sensitivity and specificity, but also was a systematic study of location of 2 test lines of the LFI test strip integrated with competitive and sandwich models.
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