期刊
JOURNAL OF DAIRY SCIENCE
卷 97, 期 5, 页码 2542-2558出版社
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2013-7459
关键词
probiotic bacteria; cell-surface component; cell-free culture supernatant; regulatory T-cell
资金
- Victoria University-Australian Postgraduate Research Award
In the current study, the relative contribution of cellsurface components (CSC) and cell-free supernatants (CFS) in the immuno-modulatory properties of 17 strains of probiotic and lactic acid bacteria (LAB) was assessed. The production of pro- and antiinflammatory cytokines including IL-2, IL-4, IL-10, IL-12 p70, IFN-gamma, tumor necrosis factor-a (TNF-alpha), and transforming growth factor-beta was measured at different time points after stimulation of buffy coat derived-peripheral blood mononuclear cells (PBMC) from healthy donors with CSC and CFS of probiotic and LAB. Results showed that CSC of probiotic and LAB strains induced production of T helper 1 and 2 type cytokines. Transforming growth factor-beta was stimulated at highest concentrations, followed by IL-10 and TNF-alpha. The CFS of all tested bacterial strains induced PBMC for significantly high levels of IL-10 secretion compared with unstimulated cells, but the values were less than lipopolysaccharide-stimulated cells. Cytokines due to CFS stimulation showed declined concentration for IL-2, TNF-a, and IL-4, and complete disappearance of IL-12, and transforming growth factor-beta in the cultured medium at 96 h of incubation. Results of cytokine data demonstrate proinflammatory TNF-a immune responses are mainly directed through cell-surface structures of probiotic and LAB, but antiinflammatory immune responses are mediated both by metabolites and cell-surfaces of these bacteria. The induction of CD4(+)CD25+ regulatory T cells after stimulation of PBMC with CSC and CFS of probiotic and LAB showed regulatory T cell activity appeared to be influenced both by the CSC and metabolites, but was principally triggered by cell surfaces of probiotic and LAB strains
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