期刊
JOURNAL OF DAIRY SCIENCE
卷 96, 期 1, 页码 63-74出版社
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2012-5896
关键词
beta-lactoglobulin; heat-induced aggregate; in vitro gastric digestibility; pepsin
In vitro gastric digestion of heat-induced aggregates of beta-lactoglobulin (beta-LG) in simulated gastric fluid was investigated using sodium dodecyl sulfate-PACE (under nonreducing and reducing conditions), native PAGE, 2-dimensional electrophoresis, and size exclusion chromatography. Heating at 90 degrees C significantly increased the digestibility of beta-LG, with a high initial digestion rate followed by a relatively constant rate of digestion at a high enzyme:substrate (E:S) ratio of 3:1. At a low E:S ratio (1:6), the rate of digestion of beta-LG was slower, and intermediate- and low-molecular-weight species could be seen. The high-molecular-weight nonnative aggregates (e.g., pentamers, tetramers, and trimers) were digested relatively rapidly, whereas some of the nonnative dimers were resistant to digestion and others were digested rapidly. The intermediate-molecular-weight species (21 to 23 kDa) were digested slowly. The digestibility of nonnative beta-LG aggregates varied significantly depending on the E:S ratio and the types of aggregate. Further investigation is necessary to identify and characterize slowly digested dimers and species of intermediate molecular weight.
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