期刊
JOURNAL OF DAIRY SCIENCE
卷 95, 期 8, 页码 4442-4446出版社
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2011-5101
关键词
bovine Prototheca mastitis; genotype; Prototheca zopfii; 18S ribosomal DNA
资金
- Academic Frontier Project of the Japanese Ministry of Education, Culture, Sports, Science and Technology (MEXT, Tokyo, Japan) [22580368]
- Nihon University
- Grants-in-Aid for Scientific Research [22580368] Funding Source: KAKEN
Prototheca zopfii causes bovine mastitis, resulting in reduced milk production and the secretion of thin watery milk with white flakes. Prototheca zopfii has been biochemically and serologically divided into at least 2 genotypes, P. zopfii genotype 1 and P. zopfii genotype 2. The latter is known to be the main causative agent of bovine protothecal mastitis. Prototheca zopfii was later reclassified into 5 varieties: var. zopfii (genotypes 1 and 2), var. 1 (formerly Prototheca blaschkeae), var. 3 (formerly P. moriformis), and var. portoricensis. In this study, the 18S ribosomal DNA sequences of diverse clinical specimens from different areas in Japan were studied to clarify the pathogenicity of P. zopfii var. zopfii. The phylogenetic tree revealed that all genotype 2 isolates were grouped in a cluster of P. zopfii var. zopfii SAG 2021(T) (type strain genotype 2), and were independent from the cluster of the genotype 1 isolates. Thus, all isolates from bovine mastitis in Japan were identified as P. zopfii genotype 2. Therefore, P. zopfii var. zopfii genotype 2 is associated with bovine mastitis.
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