期刊
JOURNAL OF DAIRY SCIENCE
卷 94, 期 11, 页码 5691-5694出版社
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2010-4086
关键词
Mediterranean river buffalo; casein; mRNA; quantification
资金
- Ministry of Agricultural and Forestry Policies (MiPAF, Rome, Italy)
Buffalo milk is characterized by the presence of all 4 casein fractions (alpha(S1), beta, alpha(S2), and kappa) encoded by the 4 tightly linked autosomal genes (CSN1S1, CSN2, CSN1S2, and CSN3, respectively). In the present paper, we report for the first time a quantitative characterization of buffalo casein transcripts and show that the 4 genes are not transcribed and translated with the same efficiency. In particular, the analysis of individual milk samples obtained from 9 Mediterranean river buffaloes showed that the most abundant casein fractions were beta (53.45%) and alpha(S1) (20.61%), followed by alpha(S2) and kappa, at 14.28 and 11.66%, respectively. Quantification of the corresponding mRNA showed that the percentage of transcripts of the 4 caseins was 16.48, 23.18, 55.87, and 4.47% for alpha(S1), beta, alpha(S2), and kappa, respectively. Translation efficiency was 0.25 for CSN1S2, 1.31 for CSN1S1, 2.39 for CSN2, and 2.69 for the CSN3 transcripts, respectively. A comparison of nucleotide sequences with the Kozak consensus sequence was also carried out to investigate if the mRNA sequences might be responsible for the observed differences.
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