期刊
JOURNAL OF DAIRY SCIENCE
卷 92, 期 7, 页码 3019-3026出版社
ELSEVIER SCIENCE INC
DOI: 10.3168/jds.2008-1744
关键词
bacteriophage; dairy product; Staphylococcus aureus; biocontrol
资金
- Ministry of Science and Technology (Spain) [SAF2004-0033, AGL2006-03659/ALI]
- FEDER Plan [CN-05-048, CN-05-049]
- Spanish Ministry of Education Ramon y Cajal Research Programme
The prevalence of bacteriophages infecting Staphylococcus aureus in dairy samples was assessed. Fourteen Staph. aureus strains were used in enrichment cultures of 75 dairy samples. All samples grew specific Staph. aureus bacteriophages. According to the host range, 8 different phages were isolated. Three of them, phages Phi H5, Phi G7, and Phi A72, were found in 89% of the samples; all the isolated phages were temperate. Phages FH5 and FA72 were used in preliminary bacterial challenge tests against Staph. aureus in milk. A phage mixture (1:1) was more effective than each single phage, most likely by preventing the survival of lysogenized cells. Phages inhibited Staph. aureus in UHT and pasteurized whole-fat milk. However, the phages were less active in semi-skimmed raw milk and little inhibition was achieved in whole, raw milk. Killing of Staph. aureus was observed at room temperature and at 37 degrees C, but not at refrigeration temperature.
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