Soluble inflammation markers in nasal lavage from CF patients and healthy controls

Background: CF sinonasal and bronchial mucosa reveal identical ion channel defects. Nasal Lavage (NL) allows non-invasive repeated sampling of airway surface liquid. We compared inflammatory mediators in NL from CF-patients and healthy controls, and in CF in relation to sinonasal pathogen colonization. Methods: From 40 CF-patients (mean age 21.8 yrs, SD 11.8 yrs.) and 52 healthy controls (mean age 31.9 yrs., SD 13.7 yrs.) NL-fluid (10 ml/nostril) concentrations of MPO, IL-8, IL-17A, sICAM-1, 1L-1 beta, IL-6, TNF-alpha, IL-10 and IL-5 were determined using cytometric bead arrays for flow cytometry. Results: CF-patients showed significantly higher MPO-concentrations in NL-fluid and higher IL-8-levels (n.s.) than controls. MPO, IL-8, IL-17A, sICAM-1, IL-1 beta and M-6 were significantly more often detectable in CF-patients than in controls. CF-patients with S. aureus colonization in both upper and lower airways had significantly elevated MPO and IL-8 levels in NL-fluid compared to S. aureus negatives. Conclusion: NL-fluid differed substantially between CF-patients and healthy controls with most promising results for IL-8 and MPO, a primarily in CF-NL assessed mediator. Further studies are required to assess effects of sample collection and processing on concentrations of inflammatory markers and to evaluate potentials of NL analysis in research and clinical routine. (C) 2012 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.