期刊
JOURNAL OF CYSTIC FIBROSIS
卷 12, 期 4, 页码 352-362出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jcf.2012.11.004
关键词
Cystic fibrosis; Neutrophils; Oxidative stress; Respiratory burst; ROS production
资金
- National Health and Medical Research Council
- Royal Hobart Hospital Research Foundation
Background: Few data exist on the functional activity of airway neutrophils in the milieu of the cystic fibrosis (CF) lung. We assessed reactive oxygen species (ROS) production by sputum neutrophils and the relationship to neutrophil viability. Identical assessments were made on peripheral blood neutrophils from CF patients. Methods: ROS production in sputum neutrophils was assessed in 31 CF patients at varying phases of clinical disease using flow cytometry. Twenty patients provided blood samples (including 16 who also provided a matched sputum sample). Neutrophil viability was determined using dual annexin V (apoptosis) and propidium iodide (necrosis) staining. Comparative peripheral blood data were obtained from 7 healthy controls. Results: ROS production was reduced in sputum compared to blood neutrophils and they demonstrated a higher level of necrosis. Subpopulations of neutrophils with different ROS production capacity were apparent in peripheral blood. Lung function was positively associated with both the proportion of blood neutrophils demonstrating increased ROS production and the proportion of apoptotic sputum neutrophils. Conclusions: CF airway neutrophils display functional exhaustion. Healthier lungs in CF appear to be associated with subpopulations, of blood neutrophils with increased oxidative burst capacity and evidence for increased neutrophil apoptosis within the airway. Crown Copyright (c) 2012 Published by Elsevier B.V. on behalf of European Cystic Fibrosis Society. All rights reserved.
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