期刊
JOURNAL OF CYSTIC FIBROSIS
卷 11, 期 1, 页码 59-62出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jcf.2011.09.001
关键词
Cystic fibrosis; Proteomic identification; MALDI-TOF MS; Non-fermenting isolates
资金
- Instituto Carlos III, Ministry of Health, Spain [CM08/166]
- Microbial Sciences Foundation (Madrid, Spain)
Identification of non-fermenting Gram-negative bacteria (NFGNB) from cystic fibrosis (CF) patients is often limited. A collection of stored NFGNB isolates (n=182) recovered from CF patients over a 15 year period was examined. The routinely reported identification during this period was compared with that obtained by MALDI-TOF MS. Isolates giving discrepant identification at the genus level were further analyzed by 16S rDNA sequencing. The MALDI-TOF MS system identified 94% of the isolates, including Burkholderia cepacia and Pandoraea spp. isolates, the latter previously misidentified as other NFGNB by conventional microbiological methods. Lack of identification by MALDI-TOF MS was associated with the absence of entries in the database. (C) 2011 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.
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