期刊
JOURNAL OF CRITICAL CARE
卷 26, 期 4, 页码 -出版社
W B SAUNDERS CO-ELSEVIER INC
DOI: 10.1016/j.jcrc.2010.09.001
关键词
Myeloperoxidase; Leukocyte count; SIRS; Sepsis
资金
- ICMR, New Delhi
Introduction: Circulating lipopolysaccharides released from bacteria may activate both neutrophils and monocytes. The activated neutrophils release myeloperoxidase (MPO), a specific enzyme with strong oxidative activity. The aim of this study was to evaluate MPO enzyme activity in plasma of critically ill patients and to check the hypothesis that these concentrations in plasma would be higher in sepsis and systemic inflammatory conditions, as neutrophils release their contents before proliferating in response to stress. Material and Methods: Blood samples were collected from 105 critically ill patients admitted to the intensive care unit, consisting of those with systemic inflammatory response syndrome (n = 42), sepsis (n = 37), and septic shock (n = 26). Plasma MPO enzyme activity was determined by o-dianisidine-H2O2 method, modified for 96-well plates. Results: The plasma MPO enzyme activity in sepsis patients was significantly higher than that in the control group (mean, 2.4 +/- 1.8 in sepsis and 1.86 +/- 1.2 nmol per milligram protein per 10 minutes in systemic inflammatory response syndrome vs 0.32 +/- 0.11 nmol per milligram protein per 10 minutes in healthy controls). Mean plasma lactate levels in sepsis (7.8 +/- 1.2 mmol/L) and shock patients (9.5 +/- 1.2 mmol/L) and cytokines like tumor necrosis factor-alpha, interleukin-8, and interleukin-1 beta were simultaneously evaluated to establish onset of inflammation and sepsis. These results show that neutrophil activation occurring during inflammation and sepsis could be detected by plasma MPO concentration. Conclusion: The plasma MPO concentrations may be a marker of the neutrophil proliferation and severity of inflammation. (C) 2011 Elsevier Inc. All rights reserved.
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