期刊
JOURNAL OF CRANIOFACIAL SURGERY
卷 23, 期 5, 页码 1333-1337出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/SCS.0b013e318260f20b
关键词
Sox10; palate; cranial neural crest; zebrafish; kaede
类别
资金
- March of Dimes Basil O'Connor Starter Scholar Award
- American Surgical Association Research Fellowship
- Shriners Hospital for Children
- Plastic Surgery Foundation
- Howard Hughes Medical Institute Medical Student Research Award
- Harvard Stem Cell Institute
Cranial neural crest cells follow stereotypic patterns of migration to form craniofacial structures. The zebrafish is a powerful vertebrate genetic model where transgenics with reporter proteins under the transcriptional regulation of lineage-specific promoters can be generated. Numerous studies demonstrate that the zebrafish ethmoid plate is embryologically analogous to the mammalian palate. A fate map correlating embryonic cranial neural crest to defined jaw structures would provide a useful context for the morphogenetic analysis of craniofacial development. To that end, the sox10:kaede transgenic was generated, where sox10 provides lineage restriction to the neural crest. Specific regions of neural crest were labeled at the 10-somite stage by photoconversion of the kaede reporter protein. Lineage analysis was carried out during pharyngeal development in wild-type animals, after miR140 injection, and after estradiol treatment. At the 10-somite stage, cranial neural crest cells anterior of the eye contributed to the median ethmoid plate, whereas cells medial to the eye formed the lateral ethmoid plate and trabeculae and a posterior population formed the mandible. miR-140 overexpression and estradiol inhibition of Hedgehog signaling resulted in cleft development, with failed migration of the anterior cell population to form the median ethmoid plate. The sox10: kaede transgenic line provides a useful tool for neural crest lineage analysis. These studies illustrate the advantages of the zebrafish model for application in morphogenetic studies of vertebrate craniofacial development.
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