期刊
JOURNAL OF CONTROLLED RELEASE
卷 180, 期 -, 页码 125-133出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2014.02.010
关键词
Allergy gene therapy; Thymulin; DNA nanoparticles; Lung remodeling
资金
- Centers of Excellence Program (PRONEX-FAPERJ)
- Brazilian Council for Scientific and Technological Development (CNPq)
- Carlos Chagas Filho, Rio de Janeiro State Research Supporting Foundation (FAPERJ)
- Coordination for the Improvement of Higher Education Personnel (CAPES)
- Sao Paulo State Research Supporting Foundation (FAPESP)
- INCT-INOFAR
- National Institutes of Health [R01EB003558, P01HL51811]
- European Community's Seventh Framework Programme (TARKINAID)
Thymulin has been shown to present anti-inflammatory and anti-fibrotic properties in experimental lung diseases. We hypothesized that a biologically active thymulin analog gene, methionine serum thymus factor, delivered by highly compacted DNA nanoparticles may prevent lung inflammation and remodeling in a mouse model of allergic asthma. The DNA nanoparticles are composed of a single molecule of plasmid DNA compacted with block copolymers of poly-L-lysine and polyethylene glycol (CK(30)PEG), which have been found safe in a human phase I/II clinical trial. Thymulin plasmids were detected in the lungs of ovalbumin-challenged asthmatic mice up to 27 days after administration of DNA nanoparticles carrying thymulin plasmids. A single dose of DNA nanoparticles carrying thymulin plasmids prevented lung inflammation, collagen deposition and smooth muscle hypertrophy in the lungs of a murine model of ovalbumin-challenged allergic asthma, leading to improved lung mechanics. In the present model of chronic allergic asthma, highly compacted DNA nanoparticles using thymulin analog gene modulated the inflammatory and remodeling processes improving lung mechanics. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据