期刊
JOURNAL OF CONTROLLED RELEASE
卷 173, 期 -, 页码 119-124出版社
ELSEVIER
DOI: 10.1016/j.jconrel.2013.10.024
关键词
Allogeneic cell; Cell viability; Diabetes; Multicellular spheroid; Silicone elastomer; Transplantation
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [23659283]
- Ritsumeikan Global Innovation Research Organization (R-GIRO)
- iCeMS Cross-Disciplinary Research Promotion Project
- Grants-in-Aid for Scientific Research [23659283, 25670257] Funding Source: KAKEN
The efficacy of cell-based therapy depends on the function and survival of transplanted cells, which have been suggested to be enhanced by spheroid formation. However, few attempts at spheroid generation from insulin-secreting cells, which may be used to treat type 1 diabetes, have been reported. We therefore developed spheroids from the mouse insulinoma cell line NIT-1 by using polydimethylsiloxane (PDMS)-based microwells with a coating of poly(N-isopropylacrylamide) (PNIPAAm). The prepared NIT-1 spheroids or dissociated NIT-1 cells were transplanted into the subrenal capsule in streptozotocin-induced diabetic mice. NIT-1 spheroids prepared using the PNIPAAm-coated PDMS-based microwells had a uniformly sized spherical structure with a diameter of 200-300 mu m. The PNIPAAm coating increased cell survival in the spheroids and the recovery of the spheroids from the microwells. In diabetic mice, the transplanted NIT-1 spheroids reduced blood glucose levels to normal values faster than dissociated NIT-1 cells did. Additionally, survival was higher among NIT-1 cells in spheroids than among dissociated NIT-1 cells 24 h after transplantation. These results indicate that insulin-secreting NIT-1 spheroids prepared using PNIPAAm-coated PDMS-based microwells are more effective for the treatment of type 1 diabetes than dissociated cells in suspension. (C) 2013 Elsevier B.V. All rights reserved.
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