期刊
JOURNAL OF CONTROLLED RELEASE
卷 164, 期 3, 页码 346-354出版社
ELSEVIER
DOI: 10.1016/j.jconrel.2012.06.029
关键词
Drug delivery; Tumour accumulation; Body distribution; HPMA copolymers; Pharmacokinetics; Renal elimination
资金
- Academy of Sciences of the Czech Republic [IAAX00500803]
- grant agency of the Czech Republic [P301/11/0325]
The molecular weight and molecular architecture of soluble polymer drug carriers significantly influence the biodistribution and anti-tumour activities of their doxorubicin (DOX) conjugates in tumour-bearing mice. Biodistribution of N-(2-hydroxypropyl) methacrylamide (HPMA) copolymer-DOX conjugates of linear and star architectures were compared in EL4 T-cell lymphoma-bearing mice. Biodistribution, including tumour accumulation, and anti-tumour activity of the conjugates strongly depended on conjugate molecular weight (MW), polydispersity, hydrodynamic radius (R-h) and molecular architecture. With increasing MW, renal clearance decreased, and the conjugates displayed extended blood circulation and enhanced tumour accumulation. The linear conjugates with flexible polymer chains were eliminated by kidney clearance more quickly than the highly branched star conjugates with comparable MWs. Interestingly, the data suggested different mechanisms of renal filtration for star and linear conjugates. Only star conjugates with MWs below 50,000 g.mo(-1) were removed by kidney filtration, while linear polymer conjugates with MWs near 70,000 g.mol(-1), exceeding the generally accepted limit for renal elimination, were detected in the urine 36-96 h after injection. Additionally, survival of tumour-bearing mice was strongly dependent on molecular weight and polymer conjugate architecture. Treatment of mice with the lower MW conjugate at a dose of 10 mg DOX eq./kg resulted in 12% long-term surviving animals, while treatment with the corresponding star conjugate enabled 75% survival of animals. (c) 2012 Elsevier B.V. All rights reserved.
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