4.8 Article

A low-invasive and effective transcutaneous immunization system using a novel dissolving microneedle array for soluble and particulate antigens

期刊

JOURNAL OF CONTROLLED RELEASE
卷 161, 期 1, 页码 10-17

出版社

ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2012.01.033

关键词

Dissolving microneedle array; Transcutaneous immunization; Vaccine; Skin; Soluble antigen; Particulate antigen

资金

  1. Program for the Promotion of Fundamental Studies in Health Sciences of the National Institute of Biomedical Innovation (NIBIO)
  2. Ministry of Education, Culture, Sports, Science, and Technology of Japan [22659033]
  3. Uehara Memorial Foundation
  4. Mochida Memorial Foundation for Medical and Pharmaceutical Research
  5. Tokyo Biochemical Research Foundation
  6. Grants-in-Aid for Scientific Research [24390041, 22659033] Funding Source: KAKEN

向作者/读者索取更多资源

Transcutaneous immunization (TCI) is a promising needle-free, easy-to-use, and low-invasive vaccination method. The hydrogel patch-based TCI system induced immune responses against soluble antigens (Ags) like toxoids, but could not induce immune responses against particulate Ags. Here, as an effective TCI system against every form of Ag, we developed a dissolving microneedle array of three lengths (200, 300, or 800 mu m) made of hyaluronate as a novel TCI device. Unlike conventional microneedles, the microneedles of our dissolving microneedle arrays dissolved in the skin after insertion. Each dissolving microneedle array effectively delivered both soluble and particulate Ags under the stratum corneum. TCI using these dissolving microneedle arrays induced effective immune responses in rats regardless of the Ag form that were comparable to conventional vaccination using subcutaneous immunization. In addition, application of these dissolving microneedle arrays caused only slight skin irritation. These findings suggest that our TCI system can simply, safely, and effectively improve protective immune responses for every vaccine Ag. (C) 2012 Elsevier B.V. All rights reserved.

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