4.8 Article

Monitoring the transport of polymeric micelles across MDCK cell monolayer and exploring related mechanisms

期刊

JOURNAL OF CONTROLLED RELEASE
卷 158, 期 3, 页码 413-423

出版社

ELSEVIER
DOI: 10.1016/j.jconrel.2011.12.018

关键词

Polymeric micelles; MDCK; Endocytosis; Transport; Transcytosis

资金

  1. National Basic Research Program of China [2009CB930300]
  2. State Key Projects [2009ZX09310-001]

向作者/读者索取更多资源

Although some formulations based on nanotechnology are already available, the transport of nanoscale particles including polymeric micelles (PMs) across epithelial cellmonolayerwas barely studied. To prove the transport of PMs across the epithelial barrier and explain the mechanisms related, a typical PM system containing a fluorescent probe Coumarin 6 (C6) was prepared and the Madin-Darby canine kidney (MDCK) cell line was used as an epithelial cell model. Four different approaches were applied to monitor the transport of PMs prepared, including the real-time and in situ imaging by a novel approach developed by our group. And the solid evidence of PMs' transport was obtained. The mechanism related was explored by different techniques. With the absence of caveolae mediated endocytosis and macropinocytosis, the clathrin mediated pathway might play a great role here, and a fraction of PMs bypassed the degradative lysosome pathway, probably due to the clathrin and caveolae independent mechanisms. Interestingly, some inhibitor was found to inhibit transcytosis of PMs significantly but not their endocytosis, suggesting different mechanisms involved in endocytosis and exocytosis. The Forster resonance energy transfer (FRET) phenomenon still existed after FRET PMs were internalized by cells. Anyhow, a multiple process with active transcellular pathway was indicated. (C) 2011 Elsevier B. V. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据