期刊
JOURNAL OF CONTROLLED RELEASE
卷 162, 期 1, 页码 242-254出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2012.04.037
关键词
Hemagglutinin; Immunopotentiating reconstituted influenza virosomes; pH-sensitive liposome; Cationic lipid; TAP (Transcription activator protein); Transfection; Immunogenicity; Subunit vaccines; Adjuvant; Microsphere; Virosomes
资金
- Department of Biotechnology
- Council of Scientific and Industrial Research
- University Grant Commission, New Delhi, India
The introduction of vaccine technology has facilitated an unprecedented multi-antigen approach to develop an effective vaccine against complex systemic inflammatory pathogens such as Plasmodium spp. that cause severe malaria. The capacity of multi subunit DNA vaccine encoding different stage Plasmodium antigens to induce CD8(+) cytotoxic T lymphocytes and interferon-gamma responses in mice, monkeys and humans has been observed. Moreover, genetic vaccination may be capable of eliciting both cell mediated and humoral immune responses. The cytotoxic T cell responses are categorically needed against intracellular hepatic stage and humoral response with antibodies targeted against antigens from all stages of malaria parasite life cycle. Therefore, the key to success for any DNA based vaccine is to design a vector able to serve as a safe and efficient delivery system. This has encouraged the development of non-viral DNA-mediated gene transfer techniques such as liposome, virosomes, microsphere and nanoparticles. Efficient and relatively safe DNA transfection using lipoplexes makes them an appealing alternative to be explored for gene delivery. Also, liposome-entrapped DNA has been shown to enhance the potency of DNA vaccines, possibly by facilitating uptake of the plasmid by antigen-presenting cells (APC). Another recent technology using cationic lipids has been deployed and has generated substantial interest in this approach to gene transfer. In this review we discussed various aspects that could be decisive in the formulation of efficient and stable carrier system(s) for the development of malaria vaccine. Published by Elsevier B. V.
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