期刊
JOURNAL OF CONTROLLED RELEASE
卷 164, 期 3, 页码 387-393出版社
ELSEVIER
DOI: 10.1016/j.jconrel.2012.06.040
关键词
siRNA; pDNA; Polyrotaxane; Polyplex; Transfection; Fluorescence
资金
- European Respiratory Society/Marie Curie Joint Research Fellowship [MC [1634]-2010]
- European Respiratory Society
- European Community's Seventh Framework Programme FP7 - Marie Curie Actions [PCOFUND-GA-2008-229571]
- 'Alexander von Humboldt' foundation, Germany
Cationic polyrotaxanes, obtained by temperature activated threading of cationic cyclodextrin derivatives onto water-soluble cationic polymers (ionenes), form metastable nanometric polyplexes with pDNA and combinations of siRNA with pDNA. Because of their low toxicity, the polyrotaxane polyplexes constitute a very interesting system for the transfection of polynucleotides into mammalian cells. The complexation of Cy3-labeled siRNA within the polyplexes was demonstrated by fluorescence correlation spectroscopy. The uptake of the polyplexes (red) was imaged by confocal fluorescence microscopy using the A549 cell line as a model (blue: nuclei, green: membranes). The results prove the potential of polyrotaxanes for further investigations involving knocking down genes of therapeutic interest. (c) 2012 Elsevier B.V. All rights reserved.
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