期刊
JOURNAL OF CONTROLLED RELEASE
卷 161, 期 1, 页码 109-115出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2012.03.028
关键词
Fire blight; Microencapsulation; Biocontrol; Pantoea agglomerans; Erwinia amylovora; Cell release
资金
- U.S. Department of Agriculture (USDA)-National Institute of Food and Agriculture (NIFA)-Special Crop Research Initiative (SCRI) [AG 2009-51181-06023]
Microencapsulation and controlled release of the biocontrol agent Pantoea agglomerans strain E325 (E325), an antagonist to the bacterial plant pathogen Erwinia amylovora that causes fire blight, a devastating disease of apple and pear, have been investigated. Uniform core-shell alginate microcapsules (AMCs), 60-300 mu m in diameter, were fabricated to encapsulate E325 within the core, along with nutrients, to preserve viability and promote proliferation. Controlled release of E325 was achieved by separately adjusting alginate concentrations in the shell and core solutions, and by modifying the AMC size. Viability of E325 was monitored via fluorescent staining, revealing either lack of or minimal stress during or after encapsulation. Proliferation of E325 within AMCs, followed by their subsequent release, and colonization activities within confines of apple flowers were studied under different encapsulation conditions using rfp-labeled E325 to obtain highly promising results. This study provided a 'proof of concept' of the successful use of a microencapsulated biocontrol agent, E325, against E. amylovora, and could serve as a model for further studies on the development of effective plant disease management strategies. (C) 2012 Elsevier B.V. All rights reserved.
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