期刊
JOURNAL OF CONTROLLED RELEASE
卷 145, 期 3, 页码 203-209出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2010.04.020
关键词
Iontophoresis; RNAse; Protein delivery; Non-invasive; Transdermal
The purpose of the study was to demonstrate the feasibility of using transdermal iontophoresis to deliver a functional protein, ribonuclease A (RNAse; 13.6 kDa), non-invasively across the skin. Iontophoretic transport experiments were conducted using porcine skin in vitro and established the effect of current density and protein concentration on delivery kinetics. A methylene blue-based assay was used to quantify RNAse transport and to simultaneously demonstrate that protein functionality was retained post-iontophoresis. The results confirmed that intact functional RNAse was indeed delivered across the skin; cumulative permeation and steady state flux after 8 h iontophoresis at 0.3 mA/cm(2) were 224.37 +/- 72.34 mu g/cm(2) and 68.28 +/- 23.87 mu g/cm(2)h, respectively. Significant amounts of protein were also deposited within the membrane (e.g., 1425.13 +/- 312.09 mu g/cm(2) at 0.3 mA/cm(2)). In addition to the evidence provided by the enzymatic assay with regards to RNAse integrity and functionality. SDS-PAGE gels and MALDI-TOF spectra were also used to characterize RNAse present in the receiver phase (MALDI-TOF spectra: RNAse control, 13.690 kDa cf. RNAse from permeation samples, 13.692 kDa). Co-iontophoresis of acetaminophen showed that, despite its molecular weight, electromigration was the predominant electrotransport mechanism, accounting for > 80% of RNAse total flux. Increasing RNAse concentration from 0.35 to 0.7 mM in the formulation did not result in a statistically significant increase in delivery. Iontophoretic transport of RNAse across human skin was statistically equivalent to that seen with porcine skin under the same conditions: cumulative permeation across human and porcine skin was 241.48 +/- 60.01 and 170.71 +/- 92.13 mu g/cm(2), respectively. laser scanning confocal microscopy was used to visualize the distribution of rhodamine B-labelled RNAse in the epidermis and dermis as a function of depth following 8 h iontophoresis (results were compared to control experiments involving passive administration of the same formulation for 8 h). Although fluorescence was localized at the skin surface following passive administration, it was visible throughout the membrane after current application. In conclusion, the results demonstrate that non-invasive transdermal iontophoresis can be used to deliver significant amounts of a structurally intact, functional protein across skin. (c) 2010 Elsevier B.V. All rights reserved.
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