期刊
JOURNAL OF CONTROLLED RELEASE
卷 137, 期 2, 页码 110-115出版社
ELSEVIER
DOI: 10.1016/j.jconrel.2009.04.005
关键词
Catalase; Pegylation; Gelatin hydrogel; Controlled release; Spontaneous metastasis
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Health and Labor Sciences Research
- Hepatitis and BSE from the Ministry of Health, Labor and Welfare of Japan
- 21st Century COE Program Knowledge Information Infrastructure for Genome Science
- US National Science Foundation [0611860]
- Office Of The Director
- Office Of Internatl Science &Engineering [0611860] Funding Source: National Science Foundation
Catalase delivery can be effective in inhibiting reactive oxygen species (ROS)-mediated acceleration of tumor metastasis. Our previous studies have demonstrated that increasing the plasma half-life of catalase by pegylation (PEG-catalase) significantly increases its potency of inhibiting experimental pulmonary metastasis in mice. In the present study, a biodegradable gelatin hydrogel formulation was used to further increase the circulation time of PEG-catalase. Implantation of In-111-PEG-catalase/hydrogel into subcutaneous tissues maintained the radioactivity in plasma for more than 14 days. Then, the effect of the PEG-catalase/hydrogel on spontaneous pulmonary metastasis of tumor cells was evaluated in mice with subcutaneous tumor of B16-111.6/Luc cells, a murine melanoma cell line stably expressing luciferase. Measuring luciferase activity in the lung revealed that the PEG-catalase/hydrogel significantly (P<0.05) inhibited the pulmonary metastasis compared with PEG-catalase solution. These findings indicate that sustaining catalase activity in the blood circulation achieved by the use of pegylation and gelatin hydrogel can reduce the incidence of tumor cell metastasis. (C) 2009 Elsevier B.V. All rights reserved.
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