期刊
JOURNAL OF CONTROLLED RELEASE
卷 139, 期 3, 页码 174-181出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jconrel.2009.06.024
关键词
Tie2; Angiogenesis; Peptide ligand; Liposome; Tumor targeting
资金
- National Science Foundation of China [30472097]
- National 863 High Technology Research and Development Program of China [2007AA021604]
Tie2 receptor is a receptor tyrosine kinase that plays important roles in vascular angiogenesis, and also highly expressed by a number of cancer cells. In this study, we reported an active targeting liposome system directed by a novel peptide ligand PHI that can improve drug efficacies specifically to Tie2 expressing cells. The PH1 peptide (TMGFTAPRFPHY) was selected by phage display library screening combined with surface plasmon resonance binding assays. It was covalently conjugated to the distal end of DSPE-PEG(2000)-Maleimide lipid and loaded onto liposome membranes as the targeting ligand. These PH1-PEG-liposomes containing the anticancer drug cisplatin were showed to bind tightly to Tie2 positive cells, mediate active endocytosis of the drug containing liposomes, and result in much higher cell specific cytoxicities than mPEG coated liposomes. They can be used not only to target vascular endothelial cells for anti-angiogenesis effects, but also to improve drug delivery and release in Tie2 expressing cancer cells. Such liposome formulation may be developed into a very useful agent for metronomic chemotherapy. (C) 2009 Elsevier B.V. All rights reserved.
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