期刊
JOURNAL OF CONTROLLED RELEASE
卷 132, 期 3, 页码 279-288出版社
ELSEVIER
DOI: 10.1016/j.jconrel.2008.06.023
关键词
Gene delivery; Ultrasound; Microbubbles; Liposomes; Nuclear entry
资金
- Ghent University (BOF)
- FWO
- European Union (MediTrans)
After administration to the body, nucleic acid containing nanoparticles (NANs) need to cross several extra- and intracellular barriers to reach the cytoplasm or nucleus of the target cells. In the last decade several groups tried to overcome these barriers by arming non-viral delivery systems with targeting moieties, polyethylene glycol chains, fusogenic peptides and so forth. However, the drawback of this upgrading strategy is that each of the encountered barriers requires a new functionality, leading to very complex multicomponent NANs. Moreover, there are currently no components available that can efficiently transport genes or NANs inside the nucleus of non-dividing cells. In this article a new, ultrasound based delivery system that possesses the capacity to simultaneously overcome several key barriers in non-viral nucleic acid delivery is presented. Additionally, a small amphiphilic compound that induces nuclear uptake of plasmid DNA and enhances non-viral gene transfer is presented. (C) 2008 Elsevier B.V. All rights reserved.
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