期刊
ACTA BIOMATERIALIA
卷 11, 期 -, 页码 233-246出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2014.09.023
关键词
Bioprinting; Silk-gelatin bioink; Cytocompatible gelation; Self-standing 3-D construct; Multi lineage differentiation
资金
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [2010-0018294, 2011-0030075]
- Indo-Korean Research Internship Program from the Indo-Korea joint program of cooperation in Science Technology [INT/ROK/IKRI-5/2013]
- Ministry of Education, Science and Technology, Republic of Korea
- National Research Foundation of Korea [2011-0030075, 2010-0018294] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Bioprinting has exciting prospects for printing three-dimensional (3-D) tissue constructs by delivering living cells with appropriate matrix materials. However, progress in this field is currently extremely slow due to limited choices of bioink for cell encapsulation and cytocompatible gelation mechanisms. Here we report the development of clinically relevant sized tissue analogs by 3-D bioprinting, delivering human nasal inferior turbinate tissue-derived mesenchymal progenitor cells encapsulated in silk fibroin-gelatin (SF-G) bioink. Gelation in this bioink was induced via in situ cytocompatible gelation mechanisms, namely enzymatic crosslinking by mushroom tyrosinase and physical crosslinking via sonication. Mechanistically, tyrosinases oxidize the accessible tyrosine residues of silk and/or gelatin into reactive o-quinone moieties that can either condense with each other or undergo nonenzymatic reactions with available amines of both silk and gelatin. Sonication alters the hydrophobic interaction and accelerates self-assembly of silk fibroin macromolecules to form beta-sheet crystals, which physically crosslink the hydrogel. However, sonication has no effect on the conformation of gelatin. The effect of optimized rheology, secondary conformations of silk-gelatin bioink, temporally controllable gelation strategies and printing parameters were assessed to achieve maximum cell viability and multilineage differentiation of the encapsulated human nasal inferior turbinate tissue-derived mesenchymal progenitor cells. This strategy offers a unique path forward in the direction of direct printing of spatially customized anatomical architecture in a patient-specific manner. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
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