期刊
JOURNAL OF COMPUTATIONAL NEUROSCIENCE
卷 34, 期 3, 页码 477-488出版社
SPRINGER
DOI: 10.1007/s10827-012-0431-7
关键词
Optogenetics; Channelrhodopsin; Neural stimulation
资金
- Wellcome Trust [097816/Z/11/A]
- UK EPSRC [EP/J501347/1]
- Royal Society Industry Fellowship
- UK EPSRC Knowledge Transfer Scheme
- EU [270324]
- Engineering and Physical Sciences Research Council [EP/J501347/1] Funding Source: researchfish
- Wellcome Trust [097816/Z/11/A] Funding Source: Wellcome Trust
Optogenetics offers an unprecedented ability to spatially target neuronal stimulations. This study investigated via simulation, for the first time, how the spatial pattern of excitation affects the response of channelrhodopsin-2 (ChR2) expressing neurons. First we described a methodology for modeling ChR2 in the NEURON simulation platform. Then, we compared four most commonly considered illumination strategies (somatic, dendritic, axonal and whole cell) in a paradigmatic model of a cortical layer V pyramidal cell. We show that the spatial pattern of illumination has an important impact on the efficiency of stimulation and the kinetics of the spiking output. Whole cell illumination synchronizes the depolarization of the dendritic tree and the soma and evokes spiking characteristics with a distinct pattern including an increased bursting rate and enhanced back propagation of action potentials (bAPs). This type of illumination is the most efficient as a given irradiance threshold was achievable with only 6 % of ChR2 density needed in the case of somatic illumination. Targeting only the axon initial segment requires a high ChR2 density to achieve a given threshold irradiance and a prolonged illumination does not yield sustained spiking. We also show that patterned illumination can be used to modulate the bAPs and hence spatially modulate the direction and amplitude of spike time dependent plasticity protocols. We further found the irradiance threshold to increase in proportion to the demyelination level of an axon, suggesting that measurements of the irradiance threshold (for example relative to the soma) could be used to remotely probe a loss of neural myelin sheath, which is a hallmark of several neurodegenerative diseases.
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