期刊
JOURNAL OF COMPARATIVE NEUROLOGY
卷 520, 期 16, 页码 3604-3616出版社
WILEY
DOI: 10.1002/cne.23115
关键词
zebrafish; neurons; lesioned spinal cord
资金
- Biotechnology and Biological Sciences Research Council (BBSRC) [BBSRC BB/H003304/1]
- Robert Packard Center for ALS Research at Johns Hopkins
- Euan MacDonald Centre for MND Research
- Biotechnology and Biological Sciences Research Council [BB/H003304/1] Funding Source: researchfish
- BBSRC [BB/H003304/1] Funding Source: UKRI
- Grants-in-Aid for Scientific Research [22657055, 21370103] Funding Source: KAKEN
In contrast to mammals, adult zebrafish regenerate neurons in the lesioned spinal cord. For example, motor neurons are generated from an olig2-expressing population of pMN-like ependymoradial glial cells in a ventrolateral position at the central canal. However, the extent of neuronal regeneration is unclear. Here we show, using a transgenic fish in which V2 interneurons are labeled by green fluorescent protein (GFP) under the control of the vsx1 promoter, that after a complete spinal cord transection, large numbers of V2 interneurons are generated in the vicinity of the lesion site. Tg(vsx1:GFP)+ cells are not present in the unlesioned spinal cord and label with the proliferation marker bromodeoxyuridine (BrdU) after a lesion. Some mediolaterally elongated Tg(vsx1:GFP)+ cells contact the central canal in a medial position. These cells likely arise from a p2-like domain of ependymoradial glial progenitor cells, indicated by coexpression of Pax6 and Nkx6.1, but not DsRed driven by the olig2 promoter in these cells. We also present evidence that Pax2+ interneurons are newly generated after a spinal lesion, whereas the generation rate for a dorsal population of parvalbuminergic interneurons is comparatively low. Our results identify the regenerative potential of different interneuron types for the first time and support a model in which different progenitor cell domains in distinct dorsoventral positions around the central canal are activated by a lesion to give rise to diverse neuronal cell types in the adult zebrafish spinal cord. J. Comp. Neurol. 520:36043616, 2012. (C) 2012 Wiley Periodicals, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据