期刊
JOURNAL OF COMPARATIVE NEUROLOGY
卷 518, 期 22, 页码 4479-4490出版社
WILEY
DOI: 10.1002/cne.22489
关键词
developmental neurogenesis; adult neurogenesis; dentate gyrus; neural stem cell; retrovirus; BrdU; cell layering
资金
- McDonnell Foundation
- Mathers Foundation
- National Institutes of Health [NS-05050217, NS-05052842, MH090258]
- National Institutes of Aging [AG-020938]
- Argentine Agency for the Promotion of Science and Technology (ANPCyT)
- Argentine Council for Scientific and Technical Research (CONICET)
- Howard Hughes Medical Institute
- Lookout Fund
New neurons are continuously added throughout life to the dentate gyrus of the mammalian hippocampus. During embryonic and early postnatal development, the dentate gyrus is formed in an outside-in layering pattern that may extend through adulthood. In this work, we sought to quantify systematically the relative position of dentate granule cells generated at different ages. We used 5'-bromo-2'-deoxyuridine (BrdU) and retroviral methodologies to birth date cells born in the embryonic, early postnatal, and adult hippocampus and assessed their final position in the adult mouse granule cell layer. We also quantified both developmental and adult-born cohorts of neural progenitor cells that contribute to the pool of adult progenitor cells. Our data confirm that the outside-in layering of the dentate gyrus continues through adulthood and that early-born cells constitute most of the adult dentate gyrus. We also found that substantial numbers of the dividing cells in the adult dentate gyrus were derived from early-dividing cells and retained BrdU, suggesting that a subpopulation of hippocampal progenitors divides infrequently from early development onward. J. Comp. Neurol. 518:4479-4490, 2010. (C) 2010 Wiley-Liss, Inc.
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