期刊
JOURNAL OF COMPARATIVE NEUROLOGY
卷 512, 期 1, 页码 6-26出版社
WILEY
DOI: 10.1002/cne.21846
关键词
GAT; VGAT; amacrine cells; horizontal cells; retina; visual system
资金
- National Institutes of Health [EY 04067, 15573]
- NATIONAL EYE INSTITUTE [R56EY004067, R01EY004067, R01EY015573] Funding Source: NIH RePORTER
Plasmalemmal and vesicular gamma-aminobutyric acid (GABA) transporters influence neurotransmission by regulating high-affinity GABA uptake and GABA release into the synaptic cleft and extracellular space. Postnatal expression of the plasmalemmal GABA transporter-1 (GAT-1), GAT-3, and the vesicular GABA/glycine transporter (VGAT) were evaluated in the developing mouse retina by using immunohistochemistry with affinity-purified antibodies. Weak transporter immunoreactivity was observed in the inner retina at postnatal day 0 (P0). GAT-1 immunostaining at P0 and at older ages was in amacrine and displaced amacrine cells in the inner nuclear layer (INL) and ganglion cell layer (GCL), respectively, and in their processes in the inner plexiform layer (IPL). At P10, weak GAT-1 immunostaining was in Muller cell processes. GAT-3 immunostaining at P0 and older ages was in amacrine cells and their processes, as well as in Muller cells and their processes that extended radially across the retina. At P10, Muller cell somata were observed in the middle of the INL. VGAT immunostaining was present at P0 and older ages in amacrine cells in the INL as well as processes in the IPL. At P5, weak VGAT immunostaining was also observed in horizontal cell somata and processes. By P15, the GAT and VGAT immunostaining patterns appear similar to the adult immunostaining patterns; they reached adult levels by about P20. These findings demonstrate that GABA uptake and release are initially established in the inner retina during the first postnatal week and that these systems subsequently mature in the outer retina during the second postnatal week. J. Comp. Neurol. 512: 6-26,2009. Published 2008 Wiley-Liss, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据