期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 428, 期 -, 页码 49-56出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2014.04.036
关键词
Amoitone B; Nanostructured lipid carriers; PEG; Apoptosis; Intracellular uptake
资金
- Natural Science Foundation of Shandong Province, China [ZR2011HM026]
- National Natural Science Foundation of China [30973646]
Amoitone B is a newly synthesized derivative of antitumor drug cytosporone B, which exhibits excellent anticancer activity in vivo. Nevertheless, the water-insolubility and short biological half-life limit its further development. In the present study, polyethylene glycol-modified, Amoitone B-loaded long circulating nanostructured lipid carriers (AmB-PEG-NLC) were prepared by the emulsion-evaporation and low temperature-solidification method. The in vitro antitumor activity and intracellular uptake of AmB-PEG-NLC in the human colon cancer SW620 cells and liver cancer HepG2 cells were evaluated in detail. MTT assay was employed to investigate the inhibition effect on cellular viability. Propidium iodide and DAPI staining were performed to visually examine the fluorescent morphology changes of the cells incubated with AmB-PEG-NLC. Flow cytometry was utilized to determine the influence of AmB-PEG-NLC on apoptosis of SW620. The intracellular uptake was observed by rhodamine B, a fluorescent maker. Cytotoxicity assay, observation of morphological changes and apoptosis examination revealed that AmB-PEG-NLC could markedly enhance the cytotoxicity of AmB against cancer cell compared to AmB solution and AmB-NLC. An increased uptake of PEG-NLC was obtained compared with NLC in SW620 cells, which might attribute to the effect of PEG. Based on these results, AmB-PEG-NLC could be a promising delivery system for AmB with effective cancer therapy. (C) 2014 Elsevier Inc. All rights reserved.
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