期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 433, 期 -, 页码 141-148出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2014.07.014
关键词
Biosensor; Tuberculosis; Nanoparticles; Electrochemical impedance spectroscopy; Cyclic voltammetry
资金
- FACEPE
- CNPq
- Rede de Nanobiotecnologia/CAPES
- CNPq [310305/2012-8, 310361/2012-5]
In this work, a genosensor for the electrochemical detection of genomic DNA from Mycobacterium tuberculosis was developed. The biosensor is based on self-assembled monolayers of mercaptobenzoic acid (MBA) and magnetite nanoparticles (Fe(3)O(4)Nps) on bare gold electrode for immobilization of DNA probe. The aim of this work was the development of a platform based on cysteine-coated magnetic Fe(3)O(4)Nps linked via the carboxylate group from MBA to the work electrode surface and subsequently to the DNA probe. The probe-genome interaction was evaluated using a [Fe(CN)(6)](4-)/[Fe(CN)(6)](3-) redox pair. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to evaluate the bioelectrochemical behavior of the sensor. Atomic force microscopy images showed Fe(3)O(4)Nps immobilized across the electrode surface. The interaction of the sensor with different genome DNA concentrations resulted in changes in the charge transfer resistance, indicating a possible use for tuberculosis detection at low concentrations (detection limit of 6 ng mu L-1). (C) 2014 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据