期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 432, 期 -, 页码 221-228出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2014.07.004
关键词
Biocompatibility; Cytokine; Graphene oxide; Lymphocyte; Macrophage
资金
- Spanish CICYT [MAT2008-00736]
- CAM [S2009/MAT-1472]
- Ministerio de Ciencia e Innovacion [CSO2010-11384-E]
- Plan Estatal I+D+i, ISCIII-Subdireccion General de Evaluacion y Fomento de la Investigacion [PI10/00650]
- Ministerio de Economia y Competitividad (MINECO), Spain
- MICINN
- CIBER-BBN
- Spanish Ministry
- Fundacao para a Ciencia e Tecnologia (FCT) Investigator Program
- Fundacao para a Ciencia e Tecnologia (FCT) [SFRH/BDP/84419/2012]
- FCT [PEst-C/EME/UI0481/2013]
Hypothesis: Graphene oxide (GO) has attracted the scientific community attention due to its novel properties and wide range of potential applications including hyperthermia cancer therapy. However, little is known about the GO effects on the immune function which involves both innate and adaptive defence mechanisms through the activation of different cell populations and secretion of several cytokines. The effect of different GO nanosheets designed for hyperthermia cancer therapy on macrophage and lymphocyte function should be determined before using GO for this application. Experiments: The effects of GO nanosheets with 1 (1-GOs) and 6 arms (6-GOs) of polyethylene glycol on RAW-264.7 macrophages and primary splenocytes (as approximation to the in vivo situation) were evaluated through the proinflammatory cytokine secretion and the modulation of cell proliferation in the presence of specific stimuli for either T-lymphocytes (concanavalin A, anti-CD3 antibody) or B-lymphocytes/macrophages (lipopolysaccharide). Findings: 6-GOs significantly increased the secretion of TNF-alpha, by RAW-264.7 macrophages without alteration of IL-6 and IL-1 beta levels. The treatment of primary splenocytes with 1-GOs and 6-GOs in the presence of concanavalin A, anti-CD3 antibody and lipopolysaccharide, produced significant dose-dependent decreases of cell proliferation and IL-6 levels, revealing weak inflammatory properties of GOs which are favourable for hyperthermia cancer therapy. (C) 2014 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据