期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 432, 期 -, 页码 77-85出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2014.06.052
关键词
Microbial adhesion; Derjaguin-Landau-Verwey-Overbeek; Parallel plate flow chamber; Drag force distribution; Weibull distribution function; Hydrodynamic force; Interfacial energy; Soft particle theory
资金
- COE program [24403]
- E-1 (Science and Engineering for Water-Assisted Evolution of Valuable Resources and Energy from Organic Wastes)
- Japan Society for the Promotion of Science (JSPS) [21241022]
- Grants-in-Aid for Scientific Research [21241022] Funding Source: KAKEN
Hypothesis: It was predicted that the colloidal behaviors of archaea and bacteria with disparate surface structure were different. In this study, the effects of the physicochemical properties of microbial cell surfaces on colloidal behavior were analyzed with Derjaguin-Landau-Verwey-Overbeek (DLVO) theory, thermodynamics, and powder technology. Experiments: Cell attachment and detachment from model substrates were directly observed using a parallel plate flow chamber. Gram-negative Escherichia coli and archaeal Methanosarcina barkeri were used as model microbial cells, and positively and negatively charged glass slides were used as model substrates. Findings: Microbial adhesion on both substrates agreed well with predictions calculated from DLVO theory, using experimental parameters. The total number of cells detached from the substrates as a function of flow rate was fit with the Weibull distribution function. In addition, the drag force required for detachment, which was estimated from the hydrodynamic forces, had a wide distribution; however, the forces became smaller with increasing ionic strength because of reduced electrostatic interactions between the cells and the substrate. M. barkeri could not be detached from positively charged substrates because it would entail a negative change in the interfacial energy of interaction. Thus adhesion was thermodynamically favored in this case. (C) 2014 Elsevier Inc. All rights reserved.
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