期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 417, 期 -, 页码 210-216出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2013.11.029
关键词
Mesopore; Surface; Ionic liquid; Immobilization; Lipase
资金
- National Science Foundation for Distinguished Young Scholars of China [21225626]
- State Key Laboratory of Materials-Oriented Chemical Engineering, National Natural Science Foundation of China for Young Scholars [20906049, 21106064]
- Key Project of National Natural Science Foundation of China [20936002]
- Hi-Tech Research and Development Program of China [2011AA02A209]
To investigate the surface effects of low cost mesoporous SiO2 on the properties of lipase, a series of modified mesoporous SiO2 was synthesized through various alkoxysilanes reacted with hydroxyl of SiO2 (C-1-SiO2, C-8-SiO2, C-16-SiO2, SH-SiO2, Ph-SiO2, NH2-SiO2). Particularly, ionic liquids as novel alkoxysilane were synthesized and subjected to modify the low cost mesoporous SiO2 (CH3IL-SiO2 and COOHIL-SiO2). The porcine pancreas lipase (PPL) was immobilized on the prepared materials. The activity assay indicated that the activation of enzymatic activity site was benefit from the longer alkyl chain of alkoxysilane because of the growing hydrophobic nature. Nevertheless, the loading of lipase decreased from 69% for PPL-C-1-SiO2 to 59% for PPL-C-16-SiO2, reflecting the growing hydrophobic property limited the immobilization yield. The characteristic of alkoxysilane group (SH-SiO2, Ph-SiO2, NH2-SiO2) was another important factor to influence immobilization efficiency and enzymatic performance besides the alkyl chain length. The immobilization efficiency of PPL-SH-SiO2, PPL-Ph-SiO2 and PPL-NH2-SiO2 maintained at least 93%. Compared with conventional alkoxysilane, the activity of PPL-CH3IL-SiO2 improved to 2.60 folds of PPL-SiO2. The immobilization efficiency of PPL-COOHIL-SiO2 was up to 97% and the relative activity was above 62% after five recycles. (C) 2013 Elsevier Inc. All rights reserved.
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