期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 394, 期 -, 页码 573-581出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2012.12.021
关键词
Self-assembly; Complexation; Solubilization; Nanoparticle; Gene delivery
资金
- National Natural Science Foundation of China [21004077, 81271695]
With an attempt to solubilize plasmid DNA (pDNA) in organic solvents, we developed nanocomplexation strategy by using block copolymer of polyethylene glycol-b-poly(L-lysine) (PEG-b-PLL). Self-assembly of PEG-b-PLL and pDNA in aqueous solution led to spherical nanocomplexes with core-shell structure when the +/- charge ratio (N/P) was higher than 1:1. This complexation had no significant impact on the helical structure of pDNA. After lyophilization, PEG-b-PLL/pDNA nanocomplexes originally formed in aqueous solution could be well dispersed in organic solvents such as THF and dichloromethane. Facilitating by nanocomplexation, pDNA could be encapsulated into PLGA nanoparticles with high efficiency, via oil-in-water emulsion solvent evaporation method. This complexation mediated pDNA solubilization in organic solvents may facilitate the development of novel nano- and micro-platforms for gene delivery. (C) 2012 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据