期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 363, 期 1, 页码 105-113出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2011.07.006
关键词
Cellular uptake; Nanoparticle-protein interaction; Confocal Raman spectroscopy; Dark-field microscopy; Quartz crystal microbalance
资金
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science, and Technology Technology [2011-0001316, 2011-0003159, 2010-0026100]
- KRCF
Intracellular uptake of serum-coated gold nanoparticles (AuNPs) in a single mammalian cell was examined in order to investigate the interactions of cell culture media and aromatic thiol-functionalized gold surfaces using micro-spectroscopic tools. The AuNPs modified by the aromatic thiols of para-aminobenzenethiol (ABT), para-hydroxy benzenethiol (HBT), and para-carboxylic benzenethiol (CBT, para-mercaptobenzoic acid) bearing NH2, OH, and COOH surface functional groups are presumed to adsorb the serum proteins as indicated from the compiled quartz crystal microbalance (QCM) data. The QCM results indicate that among the constituents, fetal bovine serum (FBS) should be the major adsorbate species on AuNPs incubated in Roswell Park Memorial Institute (RPM!) medium. The functionalized AuNPs were found to be internalized as an aggregation state in mammalian cells as evidenced by transmission electron microscopy (TEM) images. We monitored such cellular uptake behaviors of aromatic thiol-modified AuNPs using dark-field microscopy (DFM)-guided confocal surface-enhanced Raman scattering techniques in order to identify the three-dimensional localization inside the single cell. We found that the uptake amounts of ABT, HBT, and CBT were similar by counting up to 70 particles inside the cells incubated in the solution mixture of the aromatic thiol and 1,4-phenylenediisocyanide (PDIC) as a reference. This result indicates for the short aromatic thiol compounds, the AuNPs should enter the cell after the serum-coating regardless of the surface functional groups. Considering that the aromatic thiols have little effect on the serum coating, the DFM/SERS method is an effective tool for monitoring the localization of AuNPs inside a single cell. (C) 2011 Elsevier Inc. All rights reserved.
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