期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 350, 期 2, 页码 453-458出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2010.06.058
关键词
Carbon nanotube; Gold nanoparticle; Fusion protein; Electrode; Biosensor
资金
- Ministry of Education, Science and Technology [R322009000101420]
We have demonstrated the fabrication of the biosensor platforms by means of the integration of the genetically engineered fusion proteins and the uniform gold nanoparticle-deposited multi-walled nanotube hybrid (Au-MWNT-HB) films for the detection of C-reactive protein (CRP). Au-MWNT-HB films were used as a good electrochemical transducer due to their excellent electrical properties and large surface areas for the signal transduction, while the genetically engineered fusion proteins, or 6His-GBP-SpA fusion proteins, specifically bind onto the surface of the Au-MWNT-HB films and efficiently immobilize bioreceptors for the detection of CRP. As-obtained biosensor platforms were characterized by electrochemical and optical analysis and revealed better performance compared to conventional Au-based biosensors. The concept delineated herein opens a new insight into nanobiotechnology through the integration of genetically engineered biomaterials with carbon nanotube (CNT)-based nanohybrids for emerging applications. (C) 2010 Elsevier Inc. All rights reserved.
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