期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 343, 期 2, 页码 454-462出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jcis.2009.12.012
关键词
Bovine serum albumin; Aggregation; Intermediates; Protein-surfactant interaction; Fluorescence; Dynamic light scattering
资金
- Council of Scientific & Industrial Research (CSIR)
- Department of Science and Technology (DST), Government of India
- Indian Institute of Technology (IIT), Delhi
To characterize the structural changes in bovine serum albumin (BSA) on the addition of N-cetyl-N,N,N-trimethyl ammonium bromide (CTAB) and to understand the mechanism underlying aggregation of resulting protein-surfactant complex, UV-visible absorbance, steady-state fluorescence, SDS-PAGE gel electrophoresis, dynamic light scattering (DLS), and circular dichroism measurements of BSA-CTAB solutions were carried out. Changes in UV-visible absorbance and the fluorescence intensity on the addition of CTAB indicate the binding of CTAB to BSA. Changes in SDS-gel pattern of pepsin digested BSA sample, circular dichroism band at 222 nm, ANS intensity of BSA-ANS solution and hydrodynamic diameter of native state monomer on the addition of CTAB indicate that BSA unfolds in the presence of CTAB. Changes in the heme binding profile, temperature dependence of the heme binding profile and change from mixed to dynamic quenching in acrylamide quenching experiments of BSA solution on the addition of CTAB indicate that BSA unfolds to an intermediate. Turbidity and DLS measurements at 298 K, 323 K and 358 K indicate that the order of propensity to aggregate for different conformations of protein is: denatured > intermediate > native. The formation of aggregates is influenced by the nature of head and tail of the surfactant. (C) 2009 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据