4.6 Article

Performance of the Abbott RealTime™ HIV-1 assay for quantification of HIV-1 clades prevalent in China

期刊

JOURNAL OF CLINICAL VIROLOGY
卷 41, 期 4, 页码 305-309

出版社

ELSEVIER SCIENCE BV
DOI: 10.1016/j.jcv.2008.01.009

关键词

HIV-1; viral load; Abbott RealTime (TM); NucliSens EasyQ (TM)

类别

向作者/读者索取更多资源

Objective: To evaluate the performance of the Abbott RealTime(TM) HIV-1 assay for measuring viral loads of the prevalent HIV-1 clades in China. Study design: Serially diluted samples, as well as 521 clinical samples from 213 untreated HIV-1-infected individuals, 56 HIV-1-infected patients receiving ART, 60 HCV- and 57 HBV-infected patients and 135 healthy blood donors, were tested with RealTime(TM) and EaSyQ(TM). Results: Both assays exhibited linearity coefficients of >0.98. RealTime(TM) and EasyQ(TM) detected HIV-1 RNA in 87.36% and 86.99% of 269 HIV-1-seropositive samples, respectively. The correlation coefficients between the two assays for quantifying HIV-1 clades B', BC and AE were 0.884, 0.813 and 0.881, respectively, and the mean differences between the two assays for clades B', BC and AE were -0.087, 0.314 and 0.559 log(10) IU/mL, respectively. The correlation coefficient between the two assays for measuring samples from patients receiving ART was 0.945 (mean difference, 0.085 log(10) IU/mL). No false-positive samples were found among the 60 HCV-infected patients, 57 HBV-infected patients and 135 healthy blood donors. Conclusions: The Abbott RealTime(TM) HIV-1 assay shows good linearity and specificity. ART drugs and HIV-1 clades B' and BC do not affect the performance of the assay. However, clade AE may be less readily detected. (C) 2008 Elsevier B.V. All rights reserved.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据