期刊
JOURNAL OF CLINICAL PERIODONTOLOGY
卷 41, 期 3, 页码 283-294出版社
WILEY-BLACKWELL
DOI: 10.1111/jcpe.12214
关键词
additive manufacturing; biomimetic coating; melt electrospinning; periodontal regeneration
资金
- NHMRC
- Australian Research Council
- Australian Dental Research Foundation
- Portuguese Foundation for Science and Technology [SFRH/BD/62452/2009]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/62452/2009] Funding Source: FCT
Aim: This study investigated the ability of an osteoconductive biphasic scaffold to simultaneously regenerate alveolar bone, periodontal ligament and cementum. Materials and MethodsA biphasic scaffold was built by attaching a fused deposition modelled bone compartment to a melt electrospun periodontal compartment. The bone compartment was coated with a calcium phosphate (CaP) layer for increasing osteoconductivity, seeded with osteoblasts and cultured in vitro for 6weeks. The resulting constructs were then complemented with the placement of PDL cell sheets on the periodontal compartment, attached to a dentin block and subcutaneously implanted into athymic rats for 8weeks. Scanning electron microscopy, X-ray diffraction, alkaline phosphatase and DNA content quantification, confocal laser microscopy, micro computerized tomography and histological analysis were employed to evaluate the scaffold's performance. ResultsThe in vitro study showed that alkaline phosphatase activity was significantly increased in the CaP-coated samples and they also displayed enhanced mineralization. In the in vivo study, significantly more bone formation was observed in the coated scaffolds. Histological analysis revealed that the large pore size of the periodontal compartment permitted vascularization of the cell sheets, and periodontal attachment was achieved at the dentin interface. Conclusions: This work demonstrates that the combination of cell sheet technology together with an osteoconductive biphasic scaffold could be utilized to address the limitations of current periodontal regeneration techniques.
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