期刊
NEW PHYTOLOGIST
卷 207, 期 4, 页码 1170-1180出版社
WILEY
DOI: 10.1111/nph.13441
关键词
anthocyanin; betalains; Caryophyllales; lineage-specific genes; pigmentation; taxonomically restricted genes
资金
- Bill and Melinda Gates Foundation
- UKAID
- National Science Foundation [DEB 1354048, DEB 1352907]
- NERC
- Alberta Ministry of Enterprise and Advanced Education
- Alberta Innovates Technology Futures (AITF)
- Innovates Centre of Research Excellence (iCORE)
- Musea Ventures
- BGI-Shenzhen
- Natural Environment Research Council [NE/K009303/1] Funding Source: researchfish
- NERC [NE/K009303/1] Funding Source: UKRI
- Direct For Biological Sciences
- Division Of Environmental Biology [1352907] Funding Source: National Science Foundation
Betalain pigments are unique to the Caryophyllales and structurally and biosynthetically distinct from anthocyanins. Two key enzymes within the betalain synthesis pathway have been identified: 4,5-dioxygenase (DODA) that catalyzes the formation of betalamic acid and CYP76AD1, a cytochrome P450 gene that catalyzes the formation of cyclo-DOPA. We performed phylogenetic analyses to reveal the evolutionary history of the DODA and CYP76AD1 lineages and in the context of an ancestral reconstruction of pigment states we explored the evolution of these genes in relation to the complex evolution of pigments in Caryophylalles. Duplications within the CYP76AD1 and DODA lineages arose just before the origin of betalain pigmentation in the core Caryophyllales. The duplications gave rise to DODA- and CYP76AD1- isoforms that appear specific to betalain synthesis. Both betalain-specific isoforms were then lost or downregulated in the anthocyanic Molluginaceae and Caryophyllaceae. Our findings suggest a single origin of the betalain synthesis pathway, with neofunctionalization following gene duplications in the CYP76AD1 and DODA lineages. Loss of DODA- and CYP76AD1- in anthocyanic taxa suggests that betalain pigmentation has been lost twice in Caryophyllales, and exclusion of betalain pigments from anthocyanic taxa is mediated through gene loss or downregulation. [Correction added after online publication 13 May 2015: in the last two paragraphs of the Summary the gene name CYP761A was changed to CYP76AD1.]
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