期刊
NEW PHYTOLOGIST
卷 208, 期 1, 页码 13-19出版社
WILEY
DOI: 10.1111/nph.13532
关键词
cloning; DNA assembly; genetic syntax; GoldenGate; synthetic biology; Type IIS restriction endonucleases
资金
- BBSRC [BBS/E/J/000CA491, BBS/E/J/000C0662, BBS/E/J/000CA430, BB/M018180/1, BB/M004864/1, BB/I003207/1, BB/D011043/1, BBS/E/J/000CA562, BBS/E/T/000PR5885, BB/L01386X/1, BB/L017687/1, BB/L02182X/1, BB/K003712/1, BB/M028860/1, BB/M004376/1, BB/M017834/1, BB/L002957/1, BB/M017982/1, BB/K005952/1, BB/I00680X/1, BB/L014130/1, BB/L011794/1, BBS/E/J/000C0618, BB/L014106/1, BBS/E/J/000CA535, BB/L020718/1, BB/L011476/1, BBS/E/J/000CA527, BBS/E/J/000C0651, BB/M028712/1, BBS/E/J/000C0673, BBS/E/T/000PR6193] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/L01386X/1, BB/D011043/1, BB/L014130/1, BB/M017834/1, BBS/E/J/00000614, BB/M028860/1, BB/M017982/1, BBS/E/J/000C0618, BBS/E/J/00000153] Funding Source: Medline
- Biotechnology and Biological Sciences Research Council [BBS/E/J/000C0673, BBS/E/J/000CA562, BB/L017687/1, BB/M004864/1, BBS/E/J/000CA527, BB/L01386X/1, BB/L014130/1, BB/M017982/1, BB/M028712/1, BB/I003207/1, BBS/E/J/000CA430, BB/L011476/1, BBS/E/J/000C0651, BBS/E/T/000PR5885, BBS/E/T/000PR6193, BB/K005952/1, BB/M017834/1, BB/I00680X/1, BBS/E/J/000C0618, BB/M018180/1, BBS/E/J/00000153, BB/L014106/1, BB/L020718/1, BBS/E/J/000C0662, BB/M028860/1, BBS/E/J/000CA491, BBS/E/J/00000614, BB/L011794/1, BB/K003712/1, BB/D011043/1, BBS/E/J/000CA535, BB/L002957/1, BB/M004376/1] Funding Source: researchfish
- Novo Nordisk Fonden [NNF12OC0000798] Funding Source: researchfish
Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.
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