期刊
NEW PHYTOLOGIST
卷 209, 期 4, 页码 1456-1469出版社
WILEY
DOI: 10.1111/nph.13714
关键词
ABA; anion channels; Arabidopsis thaliana; nitric oxide (NO) signalling; nitrogen metabolism; potassium (K+) channels
资金
- UK Biotechnology and Biological Sciences Research Council [BB/F001673/1, BB/F001630/1]
- Discovery Early Career Researcher Award (DECRA) from the Australian Research Council (ARC) [DE140101143]
- Chinese Young 1000-Plan project
- Chinese Scholarship Council award
- Chinese National Science Foundation grant [81273487]
- Leverhulme Trust grant
- BBSRC [BB/M01133X/1, BB/F001630/1, BB/L001276/1, BB/N01832X/1, BB/M001601/1, BB/D006775/1, BB/K015893/1, BB/F001673/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/L001276/1, BB/K015893/1, BB/F001630/1, BB/N01832X/1, BB/M001601/1, BB/N018508/1, BB/D006775/1, BB/F001673/1, BB/M01133X/1] Funding Source: researchfish
Maintaining potassium (K+) nutrition and a robust guard cell K+ inward channel activity is considered critical for plants' adaptation to fluctuating and challenging growth environment. ABA induces stomatal closure through hydrogen peroxide and nitric oxide (NO) along with subsequent ion channel-mediated loss of K+ and anions. However, the interactions of NO synthesis and signalling with K+ nutrition and guard cell K+ channel activities have not been fully explored in Arabidopsis. Physiological and molecular techniques were employed to dissect the interaction of nitrogen and potassium nutrition in regulating stomatal opening, CO2 assimilation and ion channel activity. These data, gene expression and ABA signalling transduction were compared in wildtype Columbia-0 (Col-0) and the nitrate reductase mutant nia1nia2. Growth and K+ nutrition were impaired along with stomatal behaviour, membrane transport, and expression of genes associated with ABA signalling in the nia1nia2 mutant. ABA-inhibited K-in(+) current and ABA-enhanced slow anion current were absent in nia1nia2. Exogenous NO restored regulation of these channels for complete stomatal closure in nia1nia2. While NO is an important signalling component in ABA-induced stomatal closure in Arabidopsis, our findings demonstrate a more complex interaction associating potassium nutrition and nitrogen metabolism in the nia1nia2 mutant that affects stomatal function.
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