期刊
NEW PHYTOLOGIST
卷 206, 期 1, 页码 118-126出版社
WILEY
DOI: 10.1111/nph.13285
关键词
cell wall; hydrogen peroxide (H2O2); pectin; pectin methylesterase; reactive oxygen species; rice (Oryza sativa)
资金
- National Nature Science Foundation [31201150]
- Special Foundation for Scientific Research in National Research Institutes [2012RG004-3]
- National Commonwealth Agricultural Project [201203029]
- National Rice Production System Project [CARS-01-27]
- Science Foundation of Zhejiang Sci-Tech University
- Foundation of Zhejiang Provincial Top Key Discipline of Biology
- Foundation of Zhejiang Provincial Key Discipline of Botany
Hydrogen peroxide (H2O2) has been reported to increase lignin formation, enhance cell wall rigidification, restrict cell expansion and inhibit root elongation. However, our results showed that it not only inhibited rice (Oryza sativa) root elongation, but also increased root diameter. No study has reported how and why H2O2 increases cell expansion and root diameter. Exogenous H2O2 and its scavenger 4-hydroxy-Tempo were applied to confirm the roles of H2O2. Immunofluorescence, fluorescence probe, ruthenium red staining, histological section and spectrophotometry were used to monitor changes in the degree of pectin methylesterification, pectin content, pectin methylesterase (PME) activity and H2O2 content. Exogenous H2O2 inhibited root elongation, but increased cell expansion and root diameter significantly. H2O2 not only increased the region of pectin synthesis and pectin content in roottips, but also increased PME activity and pectin demethylesterification. The scavenger 4-hydroxy-Tempo reduced root H2O2 content and recovered H2O2-induced increases in cell expansion and root diameter by inhibiting pectin synthesis, PME activity and pectin demethylesterification. H2O2 plays a novel role in the regulation of pectin synthesis, PME activity and pectin demethylesterification. H2O2 increases cell expansion and root diameter by increasing pectin content and demethylesterification.
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