期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 56, 期 11, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.00895-18
关键词
Babesia; cattle; clinical babesiosis; Sri Lanka
类别
资金
- Japan Society for Promotion of Science (JSPS) [26257417, 16H05033]
- Open Partnership Joint Projects of the JSPS Bilateral Joint Research Projects
- AMED/JICA Science and Technology Research Partnership for Sustainable Development (SATREPS) project [17jm0110006h0005]
Bovine babesiosis is a serious threat to the cattle industry. We prepared blood DNA samples from 13 cattle with clinical babesiosis from the Badulla (n = 8), Jaffna (n = 3), and Kilinochchi (n = 2) districts in Sri Lanka. These DNA samples tested positive in PCR assays specific for Babesia bovis (n = 9), Babesia bigemina (n = 9), and Babesia ovata (n = 1). Twelve cattle were positive for B. bovis and/or B. bigemina. One cow was negative for the tested Babesia species but was positive for Babesia on microscopic examination; the phylogenetic positions of 18S rRNA and cytochrome oxidase subunit III gene sequences suggested that the cow was infected with Babesia sp. Mymensingh, which was recently reported from a healthy cow in Bangladesh. We then developed a novel Babesia sp. Mymensingh-specific PCR assay and obtained positive results for one other sample. Analysis of gene sequences from the cow with positive B. ovata-specific PCR results demonstrated that the animal was infected not with B. ovata but with Babesia sp. Hue-1, which was recently reported from asymptomatic cattle in Vietnam. The virulence of Babesia sp. Hue-1 is unclear, as the cow was coinfected with B. bovis and B. bigemina. However, Babesia sp. Mymensingh probably causes severe clinical babesiosis, as it was the sole Babesia species detected in a clinical case. The present study revealed the presence of two bovine Babesia species not previously reported in Sri Lanka, plus the first case of severe bovine babesiosis caused by a Babesia species other than B. bovis, B. bigemina, and Babesia divergens.
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