期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 52, 期 3, 页码 987-990出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.03247-13
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资金
- FP7 project TEMPOTEST-QC [HEALTH-2009-241742]
- FP7 project EvoTAR [HEALTH-F3-2011-2011-282004]
Thirty-two carbapenem-resistant Klebsiella pneumoniae isolates, representative of different resistance mechanisms and clonal lineages, were analyzed with the Pathogenica HAI BioDetection system, based on targeted next-generation sequencing (NGS) technology. With most strains, the system simultaneously yielded comprehensive information on relevant beta-lactam resistance determinants and accurate discrimination of clonal lineages, in a shorter time frame and in a less labor-intensive manner than currently available methods for molecular epidemiology analysis. Results supported the usefulness of targeted NGS-based technologies for similar applications.
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