4.7 Article

Role of pncA and rpsA Gene Sequencing in Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis Isolates from Southern China

期刊

JOURNAL OF CLINICAL MICROBIOLOGY
卷 52, 期 1, 页码 291-297

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AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.01903-13

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资金

  1. National Great Research Program of China [2008zx10003-009]
  2. Chinese Academy of Sciences One Hundred Talents Program (Category A)
  3. key layout program of the Chinese Academy of Sciences [KSZD-EW-Z-006]

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We sequenced pncA and rpsA genes plus flanking regions of 161 Mycobacterium tuberculosis isolates and found 10 new pncA and 3 novel rpsA mutations in pyrazinamide-resistant strains determined by the Bactec MGIT 960 system. The 3' end of rpsA might be added as the target of molecular detection of pyrazinamide susceptibility.

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